|
| Status |
Public on Oct 02, 2017 |
| Title |
A1114 |
| Sample type |
SRA |
| |
|
| Source name |
Canine fetal gonads, day 39
|
| Organism |
Canis lupus familiaris |
| Characteristics |
developmental stage: day 39 embryo
tissue: gonads
Sex: female
genotype: (-/-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Gonads were isolated by dissection of fetal tissues.
We prepared PRO-seq libraries as described in Kwak et. al. (2013) Science.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
nascent RNA
|
| Data processing |
Library strategy: PRO-Seq
We removed exact duplicate reads and trimmed a 6bp random hexamer at the 5' end of each read.
We trimmed adapter sequences and mapped PRO-seq data using BWA.
Data was converted into bigWig format.
Genome_build: canFam3
Supplementary_files_format_and_content: PRO-seq data are provided in standard bigWig and raw fastq formats.
|
| |
|
| Submission date |
Dec 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Charles G Danko |
| E-mail(s) |
dankoc@gmail.com
|
| Organization name |
Cornell University
|
| Department |
Baker Institute
|
| Lab |
Danko Lab
|
| Street address |
Hungerford Hill Rd
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL21400 |
| Series (1) |
| GSE92318 |
RSPO1 downregulation and a CFA9 Insertion are Associated with Inherited XX DSD in a Canine Model |
|
| Relations |
| BioSample |
SAMN06134810 |
| SRA |
SRX2417265 |
