|
| Status |
Public on Feb 06, 2017 |
| Title |
Trsp-Ins1KO hypothlamus 2 |
| Sample type |
RNA |
| |
|
| Source name |
Hypothlamus_Trsp-Ins1KO
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::Ins1-Cre (Trsp-Ins1KO)
tissue: Hypothlamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
|
| Label |
Cy3
|
| Label protocol |
RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner.
|
| Data processing |
The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
|
| |
|
| Submission date |
Dec 11, 2016 |
| Last update date |
Feb 06, 2017 |
| Contact name |
Akira Uruno |
| E-mail(s) |
aurno02821@gmail.com
|
| Phone |
81-22-717-8085
|
| Organization name |
Tohoku University Graduate School of Medicine
|
| Street address |
2-1 Seiryo-cho, Aoba-ku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (2) |
| GSE92235 |
Hypothalamic oxidative stress provokes leptin and insulin resistance [hypothlamus] |
| GSE92236 |
Hypothalamic oxidative stress provokes leptin and insulin resistance |
|
