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Sample GSM2421803 Query DataSets for GSM2421803
Status Public on Jan 10, 2017
Title ChIP-seq from A549 (ENCLB768PAR)
Sample type SRA
 
Source name Homo sapiens A549 immortalized cell line
Organism Homo sapiens
Characteristics antibody: EP300
line: A549
biomaterial_type: immortalized cell line
biosample encode accession: ENCBS237FNI (SAMN06121727)
dev stage: adult
Sex: male
age: 58 year
lab: Tim Reddy, Duke
link: ENCBS237FNI at ENCODE; https://www.encodeproject.org/ENCBS237FNI/
link: Derived from ENCODE donor ENCDO000AAZ; https://www.encodeproject.org/ENCDO000AAZ/
Extracted molecule genomic DNA
Extraction protocol general protocol: https://www.encodeproject.org/documents/e21fb833-cd54-4c37-a3b6-440732909433/@@download/attachment/01_ChIP_DEX_Harvest_Reddy_GGR.pdf
extraction protocol: https://www.encodeproject.org/documents/4ac7e9ce-4a92-42da-a1ab-299d4205eb96/@@download/attachment/02_ChIP_shearing_IP_reverse_v.2_Reddy_GGR.pdf
treatment protocol: https://www.encodeproject.org/documents/1bca5eae-b9ce-4df0-b401-612924dc53a4/@@download/attachment/03_KAPA_Hyper_Prep_Kit_KR0961-TDSv2.15_final_24%20rxn_Reddy_GGR.pdf
general protocol: https://www.encodeproject.org/documents/0d841e8a-a32d-416e-8f99-9db162bd09be/@@download/attachment/03_notes_Reddy_GGR.pdf
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description https://www.encodeproject.org/experiments/ENCSR686BQM/
***************
biological replicate number: 2
technical replicate number: 1
description: EP300 ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 0 hours.
experiment encode accession: ENCSR686BQM
assay title: ChIP-seq
assembly: GRCh38
possible controls: ENCSR600LQZ
encode release date: 2016-10-27
lab: Tim Reddy, Duke
library encode accession: ENCLB768PAR
size range: >200
nucleic acid starting quantity: 7
nucleic acid starting quantity units: ng
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Dec 09, 2016
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL11154
Series (1)
GSE91317 ChIP-seq from A549 (ENCSR686BQM)
Relations
BioSample SAMN06121727
SRA SRX2409905
Named Annotation GSM2421803_ENCFF197OIA_peaks_GRCh38.bed.gz
Named Annotation GSM2421803_ENCFF196RLX_signal_of_unique_reads_GRCh38.bigWig

Supplementary file Size Download File type/resource
GSM2421803_ENCFF196RLX_signal_of_unique_reads_GRCh38.bigWig 147.3 Mb (ftp)(http) BIGWIG
GSM2421803_ENCFF197OIA_peaks_GRCh38.bed.gz 177.1 Kb (ftp)(http) BED
GSM2421803_ENCLB768PAR_README.txt.gz 477 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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