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Sample GSM2400214 Query DataSets for GSM2400214
Status Public on Dec 07, 2016
Title polyA mRNA RNA-seq from A549 (ENCLB555ASA)
Sample type SRA
Source name Homo sapiens A549 immortalized cell line nuclear fraction
Organism Homo sapiens
Characteristics line: A549
biomaterial_type: immortalized cell line
description: epithelial cell line derived from a 58 year old caucasian male lung carcinoma.
biosample encode accession: ENCBS447ENC (SAMN06044714)
dev stage: adult
Sex: male
age: 58 year
lab: Thomas Gingeras, CSHL
subcellular fraction term name: nucleus
link: ENCBS447ENC at ENCODE;
link: Derived from ENCODE donor ENCDO000AAZ;
link: ENCODE dbxrefs Cellosaurus CVCL_0023;
link: ENCODE dbxrefs A549;
link: ATCC sample source;
Extracted molecule total RNA
Extraction protocol extraction method: see document
general protocol:
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
biological replicate number: 3
technical replicate number: 1
description: The libraries contained in this experiment come from the nuclear fraction of independent growths of cell line A549. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Poly-A+ RNA > 200 nucleotides in size.
experiment encode accession: ENCSR000CTM
assay title: polyA mRNA RNA-seq
assembly: GRCh38, hg19
encode release date: 2012-06-21
lab: Thomas Gingeras, CSHL
library encode accession: ENCLB555ASA
size range: >200
spikeins used: /references/ENCSR402QNO/
Data processing See GSM*_README.txt supplementary file linked below
Submission date Nov 22, 2016
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL11154
Series (1)
GSE90259 polyA mRNA RNA-seq from A549 (ENCSR000CTM)
BioSample SAMN06044714
SRA SRX2370534
Named Annotation GSM2400214_ENCFF513HBQ_plus_strand_signal_of_unique_reads_GRCh38.bigWig
Named Annotation GSM2400214_ENCFF403GIG_minus_strand_signal_of_unique_reads_GRCh38.bigWig
Named Annotation GSM2400214_ENCFF405QHV_plus_strand_signal_of_all_reads_GRCh38.bigWig
Named Annotation GSM2400214_ENCFF196ERN_minus_strand_signal_of_all_reads_GRCh38.bigWig

Supplementary file Size Download File type/resource
GSM2400214_ENCFF196ERN_minus_strand_signal_of_all_reads_GRCh38.bigWig 239.0 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF376QTI_minus_strand_signal_of_all_reads_hg19.bigWig 239.4 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF403GIG_minus_strand_signal_of_unique_reads_GRCh38.bigWig 222.0 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF405QHV_plus_strand_signal_of_all_reads_GRCh38.bigWig 247.7 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF407SZF_transcript_quantifications_GRCh38.tsv.gz 8.0 Mb (ftp)(http) TSV
GSM2400214_ENCFF492OGP_gene_quantifications_hg19.tsv.gz 2.5 Mb (ftp)(http) TSV
GSM2400214_ENCFF505GJJ_minus_strand_signal_of_unique_reads_hg19.bigWig 220.8 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF513HBQ_plus_strand_signal_of_unique_reads_GRCh38.bigWig 231.7 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF578YTC_transcript_quantifications_hg19.tsv.gz 7.9 Mb (ftp)(http) TSV
GSM2400214_ENCFF630HJD_plus_strand_signal_of_all_reads_hg19.bigWig 247.9 Mb (ftp)(http) BIGWIG
GSM2400214_ENCFF853JHY_gene_quantifications_GRCh38.tsv.gz 2.6 Mb (ftp)(http) TSV
GSM2400214_ENCFF960KVD_plus_strand_signal_of_unique_reads_hg19.bigWig 230.8 Mb (ftp)(http) BIGWIG
GSM2400214_ENCLB555ASA_README.txt.gz 1.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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