|
| Status |
Public on Nov 01, 2007 |
| Title |
CaMV::DME stamen |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis (C24 ecotype) CaMV::DME stamen from stages 13 and 14 flowers
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis Columbia glabrous ecotype (C24)
|
| Growth protocol |
Plants were grown in standard green house conditions using a 16:8 light-dark cycle.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol (Invitrogen) and poly(A)+ RNA was selected using Dynabeads mRNA Purification kit (Dynal Inc) according to the manufacturers.
|
| Label |
biotin
|
| Label protocol |
Fifteen micrograms of total RNA was poly(A)+ selected using Dynabeads mRNA purification kit (Dynal Inc.). Double-stranded cDNAs were generated using Superscript Choice System (Invitrogen). Biotinylated cRNAs were synthesized with the ENZO BioArray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences).
|
| |
|
| Hybridization protocol |
Following fragmentation, fifteen micrograms of cRNA were hybridized for 16 hr at 45°C with the GeneChip ATH1 Array. GeneChips were washed and stained using the EukGE-WS2V4_450 protocol in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G System.
|
| Description |
CaMV::DME Columbia glabrous stamens
|
| Data processing |
The data were analyzed with the Affymetrix GeneChip Operating System 1.3 (GCOS 1.3) using default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was set to 500.
|
| |
|
| Submission date |
Oct 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bob Goldberg |
| E-mail(s) |
bobglab@mcdb.ucla.edu
|
| Phone |
310-825-3270
|
| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
|
| Street address |
610 Charles E Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE9408 |
Identification of putative Arabidopsis DEMETER target genes by GeneChip Analysis |
|
| Relations |
| Reanalyzed by |
GSE119083 |
