|
| Status |
Public on Aug 01, 2017 |
| Title |
I363V Treg_3 |
| Sample type |
RNA |
| |
|
| Source name |
hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3I363V:hCD2/WT:GFP mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3I363V:hCD2/WT:GFP mice
|
| Treatment protocol |
None
|
| Growth protocol |
ex vivo isolated cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
|
| |
|
| Hybridization protocol |
The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
freshly isolated cells
|
| Data processing |
Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
|
| |
|
| Submission date |
Nov 08, 2016 |
| Last update date |
Aug 01, 2017 |
| Contact name |
Shohei Hori |
| E-mail(s) |
shohei.hori@riken.jp
|
| Phone |
+81-45-503-7069
|
| Organization name |
RIKEN Center for Integrative Medical Sciences
|
| Lab |
Laboratory for Immune Homeostasis
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE89654 |
Expression data from Treg cells expressing mutant FoxP3 |
| GSE89744 |
Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells |
|
