|
| Status |
Public on Dec 20, 2007 |
| Title |
Embryo 48 h; Seed germination; Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Barley Embryos from germinating seeds, 48h imbibed
|
| Organism |
Hordeum vulgare |
| Characteristics |
Genotype: Barke cultivar
Age: 48h after imbibition
|
| Treatment protocol |
endosperm+aleurone (e/a) and embryo+scutellum (em) fractions were dissected from germinating seeds 0, 24, 48 and 72 after imbibition
|
| Growth protocol |
Seeds were germinated on a moist filter paper in Microclima 1000 growth chambers (Snijders Scientific B.V., Tilburg, Holland) with 16 h light (17oC; 337-377 mmol m-2 s-1 light intensity measured by Sky Quantium light sensor) and 8 h darkness (12oC), 80% humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PURESCRIPT® RNA isolation kits (Gentra Systems, Inc., Minneapolis, MN) , followed by Rneasy spincolumn cleanup
|
| Label |
Probe synthesis, labelling and hybridisation were performed according to manufacturer’s protocols (Affymetrix, Santa Clara, CA) at the UCI MicroArray Core Facility, Irvine, Ca.]
|
| Label protocol |
The BioArray High-Yield RNA Transcript Labeling Kit (Enzo Diagnostics) was then used to synthesize biotin-labeled cRNA from template cDNA by in vitro transcription.
|
| |
|
| Hybridization protocol |
10 µg labeled, fragmented cRNA was then hybridized at 45°C with rotation for 16 hours in an Affymetrix GeneChip Hybridization Oven 320 on Affymetrix Barley1 Genechip arrays. The arrays were washed and stained using streptavidin phycoerythrin on an Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from germinationg barley embryo 48h after imbibition
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 18, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Björn Usadel |
| E-mail(s) |
usadel@mpimp-golm.mpg.de
|
| Organization name |
Max Planck Institute of Molecular Plant Physiology
|
| Street address |
Am Mühlenberg 1
|
| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1340 |
| Series (1) |
| GSE9365 |
Expression data from barley maturing and germinating grains |
|
