|
| Status |
Public on Jan 19, 2008 |
| Title |
F1 hybrid (domesticus mother x musculus father) 2, biological rep2, testis |
| Sample type |
RNA |
| |
|
| Source name |
testis
|
| Organism |
Mus musculus musculus x M. m. domesticus |
| Characteristics |
Gender: male, age: 50 days, tissue: testis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Ambion MessageAmp™ II aRNA Amplification Kit from 1ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12ug of cRNA were hybridized for 16-18 hr at 45 °C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000 scanner.
|
| Description |
Gene expression data from M. m. musculus father x M. m. domesticus mother F1 hybrids, testis
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Bioconductor default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 660 for brain and testis and to 800 for liver.
|
| |
|
| Submission date |
Oct 16, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ruth Rottscheidt |
| E-mail(s) |
ruth.rottscheidt@uni-koeln.de
|
| Organization name |
University of Cologne
|
| Department |
Institute for Genetics
|
| Lab |
Tautz Harr Mouse Evolutionary Genomics
|
| Street address |
Zuelpicher Strasse 47
|
| City |
Cologne |
| ZIP/Postal code |
50674 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9338 |
Expression data from Mus musculus subspecies and their F1 hybrids for 3 tissues (brain, liver, testis) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
