|
| Status |
Public on Oct 15, 2016 |
| Title |
606 2015 Lgr5 Ep- MIXED |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted tumor cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Lgr5CreER/KrasG12D/p53KO/Rosa-YFP
treatment: 10mg TAM
isolation: FACS
staining: Epcam-/Lin-/DAPI-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
qiagen kit
|
| Label |
Biotin
|
| Label protocol |
25 ng total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 8 µg of cDNA were subsequently fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Affymetrix Mouse Genome 430PM strip arrays for 16 hours at 45ºC.Washing and staining of microarrays was performed using the GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
|
| Data processing |
RMA normalization method and batch effect removal with ComBat
|
| |
|
| Submission date |
Oct 14, 2016 |
| Last update date |
Oct 17, 2016 |
| Contact name |
Cédric Blanpain |
| E-mail(s) |
cedric.blanpain@ulb.ac.be
|
| Organization name |
ULB
|
| Street address |
Route de Lennik 808
|
| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE71621 |
Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition |
| GSE88762 |
Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 2] |
|
