|
| Status |
Public on Sep 13, 2016 |
| Title |
DEP treated mouse ear Replicate 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DEP_ear
|
| Organism |
Mus musculus |
| Characteristics |
treatment: DEP
tissue: Ear
treatment: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations.
|
| Label |
Cy5
|
| Label protocol |
The RNA samples were labeled using a Low Input Quick Amp Labeling Kit (Agilent Technologies), in accordance with the manufacturer’s protocol.
|
| |
|
| Channel 2 |
| Source name |
CON_ear
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Ear
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations.
|
| Label |
Cy3
|
| Label protocol |
The RNA samples were labeled using a Low Input Quick Amp Labeling Kit (Agilent Technologies), in accordance with the manufacturer’s protocol.
|
| |
|
| |
| Hybridization protocol |
Labeled cRNA was hybridized on Mouse (V2) Gene Expression Microarray 4x44K (ID G2519-026655; Agilent Technologies) followed by manual washing, according to the manufacturer’s procedures.
|
| Scan protocol |
Scanned on an Agilent SureScan microarray scanner.
|
| Description |
Biological replicate 4 of 4. DEP treated mouse ear.
|
| Data processing |
Probe signals were quantified using Feature Extraction software (version 10.10.1.1; Agilent) and data were nomalized using Subio platform v1.16.4376 (Subio Inc. Japan)
|
| |
|
| Submission date |
Sep 12, 2016 |
| Last update date |
Sep 13, 2016 |
| Contact name |
Hyo-Jeong Kim |
| E-mail(s) |
hd414@naver.com
|
| Organization name |
Dongguk University
|
| Street address |
32, Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Republic of Korea
|
| City |
Goyang |
| ZIP/Postal code |
10326 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE86844 |
Identification of novel potential biomarkers and signaling pathways related to otitis media induced by diesel exhaust particle in in vivo system via transcriptomic analysis |
|
