|
| Status |
Public on Jan 31, 2019 |
| Title |
P21_R2 |
| Sample type |
SRA |
| |
|
| Source name |
Purkinje cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cerebellum - Purkinje cells
strain: C56/BL6
developmenttal timepoint: postnatal day P21
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purkinje cells were laser microdissected, flash frozen on dry ice, and RNA was harvested using the RNeasy Micro Kit (Qiagen).
cDNA libraries were prepared using the SMARTer Ultra Low RNA Kit for Illumina sequencing (Clontech), followe by NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Trimmed for SMARTer adapter sequences with Cutadapt v1.2.1
Aligned with GSNAP 2012-07-20
Merge BAM files across sequencing lanes
Uniquely aligning reads counted with HTSeq 0.5.3p9
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: txt files of HTSeq count outputs over Ensembl 72 GRCm38.p1 gene models
|
| |
|
| Submission date |
Sep 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Esther Becker |
| E-mail(s) |
esther.becker@dpag.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Street address |
Sherrington Road
|
| City |
Oxford |
| ZIP/Postal code |
OX13PT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE86824 |
RNA Sequencing of mouse Purkinje cells across postnatal development |
|
| Relations |
| BioSample |
SAMN05762893 |
| SRA |
SRX2159688 |
