|
| Status |
Public on Jan 01, 2018 |
| Title |
MDA-shRNA-morgana-replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-treated1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
genotype/variation: sh-morgana infected
|
| Treatment protocol |
Cells were previously infected with a lentiviral PGIPZ vector containing shRNA targeting Morgana (MDA-sh) or an empty vector as control (MDA)
|
| Growth protocol |
Cells were maintaned in DMEM medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Euroclone, Milano, Italy) and 1% penicillin streptomycin (Thermo Fisher Scientific, Waltham, MA, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol AND Absolute RNA miRNA kit (Agilent) following manufacturer's instructions;
|
| Label |
Cy3
|
| Label protocol |
1 microgram of total RNA was amplified with ammino-allyl message amp II kit (Ambion) and labeled with Cy3 (GE-Heathcare), using the indirect labelling protocol;
|
| |
|
| Hybridization protocol |
200 ng of labeled RNA were hybridized on Agilent SurePrint G3 Human Gene Expression 8x60K glass arrays;
|
| Scan protocol |
The glass slide was scanned using an Agilent scanner and images were analyzed using Agilent Feature Extraction Software (version 10.7.3.1);
|
| Data processing |
raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis);
|
| |
|
| Submission date |
Sep 06, 2016 |
| Last update date |
Jan 01, 2018 |
| Contact name |
giovanna chiorino |
| E-mail(s) |
giovanna.chiorino@gmail.com
|
| Organization name |
Fondo Edo Tempia
|
| Department |
Cancer Genomics
|
| Street address |
via malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE86463 |
The IKK complex requires Morgana to drive NF-kB dependent breast cancer metastatisation |
|
