|
| Status |
Public on Feb 01, 2018 |
| Title |
Testo_100nM_IP_AR_2 |
| Sample type |
SRA |
| |
|
| Source name |
Neural Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: ES-derived neural stem cells
treatment: Testosterone 100nM 4 hours
antibody: AR (N-20 Santa Cruz sc-816)
|
| Treatment protocol |
Cells were placed in depleted medium (phenol-red free medium without N2 and B27) during 48h hours before androgen treatments and thus treated with DMSO, testosterone, DHT 10nM or 100nM during 4h hours.
|
| Growth protocol |
ES cell–derived hNSC cells were routinely maintained on poly-ornithine and laminin-coated dishes in Dulbecco's modified Eagle's medium/F12 Glutamax and Neurobasal medium (1:1) supplemented with N2, B27 and 50µM 2-mercaptoethanol containing brain-derived growth factor (20ng/mL), fibroblast growth factor-2 (10ng/mL) and epidermal growth factor (10ng/mL).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cellular lysates were isolated by incubating cells pellets in SDS lysis buffer, sonicated and AR-DNA complexes were isolated with antibody.
Libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer's protocol (V12.10)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
ChIP-seq reads were aligned to the human reference genome (hg19) using Bowtie v0.12.8
Peak calling was performed using MACS v1.4.2
Peaks were annotated relative to genomic features using Homer
Peaks were annotated using Homer v4.7.2 with annotation extracted from Ensembl v75
Genome_build: hg19
Supplementary_files_format_and_content: wig
|
| |
|
| Submission date |
Sep 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Angélique Quartier |
| E-mail(s) |
a.quartier@inoviem.com
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE86454 |
Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism [hNSCs] |
| GSE86458 |
Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism |
|
| Relations |
| BioSample |
SAMN05730832 |
| SRA |
SRX2140302 |
