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Sample GSM2302958 Query DataSets for GSM2302958
Status Public on Feb 01, 2018
Title Testo_100nM_IP_AR_2
Sample type SRA
Source name Neural Stem Cells
Organism Homo sapiens
Characteristics cell type: ES-derived neural stem cells
treatment: Testosterone 100nM 4 hours
antibody: AR (N-20 Santa Cruz sc-816)
Treatment protocol Cells were placed in depleted medium (phenol-red free medium without N2 and B27) during 48h hours before androgen treatments and thus treated with DMSO, testosterone, DHT 10nM or 100nM during 4h hours.
Growth protocol ES cell–derived hNSC cells were routinely maintained on poly-ornithine and laminin-coated dishes in Dulbecco's modified Eagle's medium/F12 Glutamax and Neurobasal medium (1:1) supplemented with N2, B27 and 50µM 2-mercaptoethanol containing brain-derived growth factor (20ng/mL), fibroblast growth factor-2 (10ng/mL) and epidermal growth factor (10ng/mL).
Extracted molecule genomic DNA
Extraction protocol Cellular lysates were isolated by incubating cells pellets in SDS lysis buffer, sonicated and AR-DNA complexes were isolated with antibody.
Libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer's protocol (V12.10)
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Data processing ChIP-seq reads were aligned to the human reference genome (hg19) using Bowtie v0.12.8
Peak calling was performed using MACS v1.4.2
Peaks were annotated relative to genomic features using Homer
Peaks were annotated using Homer v4.7.2 with annotation extracted from Ensembl v75
Genome_build: hg19
Supplementary_files_format_and_content: wig
Submission date Sep 06, 2016
Last update date May 15, 2019
Contact name Angélique Quartier
Organization name IGBMC
Street address 1, rue Laurent Fries
City Illkirch
ZIP/Postal code 67400
Country France
Platform ID GPL16791
Series (2)
GSE86454 Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism [hNSCs]
GSE86458 Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism
BioSample SAMN05730832
SRA SRX2140302

Supplementary file Size Download File type/resource
GSM2302958_Testo_100nM_IP_AR_2_peaks.wig.gz 215.4 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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