|
| Status |
Public on Oct 11, 2007 |
| Title |
PU.1 ChIP versus IgG ChIP |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
PU.1 ChIP from RAW264.7 macrophages
|
| Organism |
Mus musculus |
| Characteristics |
RAW264.7 macrophages
PU.1 ChIP from RAW264.7 macrophages
|
| Treatment protocol |
ChIP analysis was performed as described in Weigelt et al. 2007, Journal of Leukocyte Biology doi:10.1189/jlb.0707447
|
| Growth protocol |
RAW264.7 cells were cultured in DMEM /10% FCS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was prepared as described in Weigelt et al. 2007, Journal of Leukocyte Biology doi:10.1189/jlb.0707447
|
| Label |
biotin
|
| Label protocol |
Affymetrix Chromatin Immunoprecipitation Assay Protocol
|
| |
|
| Channel 2 |
| Source name |
IgG ChIP from RAW264.7 macrophages
|
| Organism |
Mus musculus |
| Characteristics |
RAW264.7 macrophages
IgG ChIP from RAW264.7 macrophages
|
| Treatment protocol |
ChIP analysis was performed as described in Weigelt et al. 2007, Journal of Leukocyte Biology doi:10.1189/jlb.0707447
|
| Growth protocol |
RAW264.7 cells were cultured in DMEM /10% FCS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was prepared as described in Weigelt et al. 2007, Journal of Leukocyte Biology doi:10.1189/jlb.0707447
|
| Label |
biotin
|
| Label protocol |
Affymetrix Chromatin Immunoprecipitation Assay Protocol
|
| |
|
| |
| Hybridization protocol |
Affymetrix Chromatin Immunoprecipitation Assay Protocol
|
| Scan protocol |
Affymetrix Chromatin Immunoprecipitation Assay Protocol
|
| Description |
3 biological replicates of PU.1 and IgG Immunoprecipitation were performed each
|
| Data processing |
Affymetrix GCOS processed image files to generate CEL files. The Affymetrix CEL files were analyzed with Genomatix Chipinspector using SAM analysis with multiple T-test and FDR to retrieve signficantly enriched promoters.
|
| |
|
| Submission date |
Sep 11, 2007 |
| Last update date |
Jan 05, 2012 |
| Contact name |
Thomas Langmann |
| E-mail(s) |
thomas.langmann@klinik.uni-regensburg.de
|
| Phone |
+49-941-944-5423
|
| Fax |
+49-941-944-5402
|
| Organization name |
Institute of Human Genetics
|
| Department |
University of Regensburg
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5811 |
| Series (1) |
| GSE9011 |
Genome-wide in vivo binding analysis for PU.1 in RAW264.7 macrophages |
|
