|
Status |
Public on Jul 26, 2017 |
Title |
WT_NT_Input_Rep1 |
Sample type |
SRA |
|
|
Source name |
WT_NT_Input
|
Organism |
Caenorhabditis elegans |
Characteristics |
developmental stage: L3-L4 mix treated with: none; no treatment (NT) tissue: whole body chip antibody: none
|
Treatment protocol |
Worms were treated with or without antimycin A.
|
Growth protocol |
Worms were grown on NGM agar, and maintained at 22℃.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Worms were collected when became L3-L4 mix and handled with standard ChIP protocol. Purified DNA was used for DNA library construction using NEBNext Ultra DNA Library Prep Kit for Illumina.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
ChIP-seq input for no treatment (NT) worms .
|
Data processing |
Basecalls performed using CASAVA version 1.8.2 ChIP-seq reads were aligned to the UCSC ce10 genome assembly using bwa 0.7.13 with default settings Only the reads with no more than 4% mismatches and uniquely mapped to reference genome were kept by using Bash Shell. Peaks were called using MACS2 (version 2.1.0). Genome_build: ce10 Supplementary_files_format_and_content: bigwig files were generated using deepTools
|
|
|
Submission date |
Aug 19, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Yong Peng |
E-mail(s) |
yongp@outlook.com
|
Organization name |
University of Chicago
|
Department |
Department of Chemistry
|
Lab |
Chuan He
|
Street address |
929 E 57th Street
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60615 |
Country |
USA |
|
|
Platform ID |
GPL18245 |
Series (1) |
GSE85835 |
Transgenerational inheritance of mitochondrial stress adaptation in C. elegans |
|
Relations |
BioSample |
SAMN05595760 |
SRA |
SRX2035138 |