|
| Status |
Public on Aug 17, 2016 |
| Title |
Eutopic Endometrium Sample- Patient 04 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Eutopic endometrial tissue - EndometriosisPatient
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA extracted and purification followed manufacturer's instructions (MasterPure™ DNA Purification Kit- Epicentre, Illumina).
|
| Label |
Cy5
|
| Label protocol |
1ug of gDNA for each sample labeled (test and control) were required. The references were labelled with Cy3-dUTP and endometrium DNA samples were labelled with Cy5-dUTP. The SureTag Complete DNA Labeling kit was used following manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
Commercial DNA Control Promega
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA extracted and purification followed manufacturer's instructions (MasterPure™ DNA Purification Kit- Epicentre, Illumina).
|
| Label |
Cy3
|
| Label protocol |
1ug of gDNA for each sample labeled (test and control) were required. The references were labelled with Cy3-dUTP and endometrium DNA samples were labelled with Cy5-dUTP. The SureTag Complete DNA Labeling kit was used following manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
The hybridization was performed according Agilent's protocol for a-CGH 2x400K. After hybridization, slides were washed sequential
|
| Scan protocol |
Arrays were scanned using an Agilent microarray scanner (Agilent Technologies, Inc), and processed using Agilent’s Feature Extraction software followed manufacturer's instructions.
|
| Description |
Women with diagnosis of severe endometriosis and familial history of the disease.
|
| Data processing |
Agilent Feature Extraction Software (Protocol: CGH_107_sep09; Template:021850_D_F_20111015) was used for background subtraction and normalization.
|
| |
|
| Submission date |
Aug 16, 2016 |
| Last update date |
Aug 17, 2016 |
| Contact name |
Flavia Gaona de Oliveira Gennaro |
| E-mail(s) |
flaviagaona@usp.br
|
| Phone |
+5516991567254
|
| Organization name |
USP
|
| Department |
Genetics
|
| Lab |
Human Molecular Cytogenetics
|
| Street address |
Av Bandeirantes, 3900, Depto. Genética, Bloco C
|
| City |
Ribeirão Preto |
| State/province |
São Paulo |
| ZIP/Postal code |
14049900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL9777 |
| Series (1) |
| GSE85701 |
Identification of rare copy number polymorphic gains at 3q12.2 and 19q13.2 identifies candidate genes for familial endometriosis |
|
