|
| Status |
Public on Jun 10, 2017 |
| Title |
CHC001 PDX ET-743 - replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
CHC001 PDX ET-743 - replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CHC001 PDX ET-743 (0.15 mg/Kg weekly i.v., three administrations)
treatment: ET-743 at the dose of 0.15 mg/Kg/weekly for three weeks
|
| Treatment protocol |
For in vitro experiment: 24 hours at 5 nM of ET-743. In Vivo: 3 weeks of treatement with ET-743 at the dose of 0.15 mg/Kg/weekly i.v.
|
| Growth protocol |
MT-CHC01 cells were cultured in KODMEM-F12 with 10% fbs, glutammine, Hepes and P/S
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol AND Absolute RNA miRNA kit (Agilent) following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA were amplified and labeled using Low Input Quick Amp Labeling Kit, one-color kit (Agilent Technologies);
|
| |
|
| Hybridization protocol |
Six hundred ng of labeled RNA were hybridized on SurePrint G3 Human Gene Expression 8x60K v2 glass arrays;
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 10.7).
|
| Data processing |
raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis).
|
| |
|
| Submission date |
Jul 27, 2016 |
| Last update date |
Jun 10, 2017 |
| Contact name |
giovanna chiorino |
| E-mail(s) |
giovanna.chiorino@gmail.com
|
| Organization name |
Fondo Edo Tempia
|
| Department |
Cancer Genomics
|
| Street address |
via malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE84918 |
Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [gene expression] |
| GSE84939 |
Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line |
|
