treatment group: Saline tissue: brain tissue compartment: Striatum Sex: male age: 80-90 days old strain: Long Evans
Treatment protocol
Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule
total RNA
Extraction protocol
Trizol protocol for total RNA following manufacturer's protocol
Label
Cy3
Label protocol
Agilent one color protocol for Cy3 following manufacturer's protocol
Hybridization protocol
Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol
Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing
Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
