|
| Status |
Public on Jan 01, 2017 |
| Title |
Clock Mutant - 21 mon, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Clock - 21 mo
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl/6J
genotype/variation: Clock delta 19/delta 19
tissue: Ventricular
|
| Treatment protocol |
The hearts were collected and snap frozen in liquid Nitrogen.
|
| Growth protocol |
The mice were collected at 4 or 21 months of age at ZT 6 ( 6 hours into the light phase)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy method according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with GeneSpring GX Software v. 11.5 using default Affymetrix settings.
|
| |
|
| Submission date |
Jul 07, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Faisal James Alibhai |
| Organization name |
University of Guelph
|
| Department |
Biomedical Sciences
|
| Lab |
Martino Lab
|
| Street address |
50 Stone Rd East
|
| City |
Guelph |
| State/province |
ON |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE84151 |
miRNA Expression data from murine hearts of transgenic Clock delta 19/delta 19 mice (Clock mice) and wild type (WT) mice at 4 and 21 months of age |
|
