|
| Status |
Public on Dec 12, 2016 |
| Title |
TH-TRPA1 18C |
| Sample type |
SRA |
| |
|
| Source name |
TH-TRPA1 18C
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: TH-GAL4;UAS-EGFP;UAS-dTrpA1
age: young
temperature: 18C
time (min): 0
Sex: pooled male and female
cell type: DA neurons
|
| Treatment protocol |
Flies were reared at 18C and temperature shift to 29C for stimulation for 60min.
|
| Growth protocol |
Drosophila were reared on standard cornmeal/agar medium supplemented with yeast under 12 hour LD cycles at 25 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Brains were dissected for neuron sorting and cells were stored in lysis buffer (Ambion, Dynabead mRNA extraction kit) at -80C.
refer to Abruzzi et al., 2014
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Description |
Sample 58
|
| Data processing |
cl2fastq v2.16.0.10 is used for base-calling
tophat for alignment
cufflinks for quantification
Genome_build: dm3
Supplementary_files_format_and_content: [fpkm_tracking] files generated by Cufflinks include FPKM values for each gene
|
| |
|
| Submission date |
Jul 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiao Chen |
| E-mail(s) |
chxiao@brandeis.edu
|
| Organization name |
Brandeis University
|
| Department |
Biology
|
| Lab |
Rosbash Lab
|
| Street address |
415 South St
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02451 |
| Country |
USA |
| |
|
| Platform ID |
GPL15334 |
| Series (2) |
| GSE83969 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [DA_PDF] |
| GSE83976 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila |
|
| Relations |
| Reanalyzed by |
GSM3281563 |
| BioSample |
SAMN05346380 |
| SRA |
SRX1892886 |
