|
| Status |
Public on Oct 06, 2016 |
| Title |
Vehicle_3357 |
| Sample type |
SRA |
| |
|
| Source name |
ventricle cardiac myocyte
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: neonatal
treatment: vehicle control
genotype: wildtype
|
| Treatment protocol |
One day after isolation, NRVMs were treated with control micro RNA mimic or miR-9 mimic (25nM) then 24 hrs after miR treatment, the cells were washed and treated with phenylepherine (10µM) or vehicle. After 48 hours of stimulation, cells were harvested for RNA seq analysis
|
| Growth protocol |
Cardiomyocytes isolated from the ventricles of neonatal rat hearts were plated on 10cm plastic dishes coated with 0.2% gelatin at a density of 160,000 cells/ml in 12 ml of media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from NRVMs using the High Pure RNA Isolation Kit (Roche)
NA was submitted to the WITG for library preparation (TruSeq Stranded mRNA Library Prep Kit, Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
FASTq files were aligned with Tophat2 and quantified using Cufflinks
Genome_build: rn4
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Jun 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Timothy McKinsey |
| E-mail(s) |
TIMOTHY.MCKINSEY@UCDENVER.EDU
|
| Phone |
303-724-5479
|
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Division of Cardiology
|
| Lab |
McKinsey
|
| Street address |
12700 E. 19th Ave (8450E)
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL14844 |
| Series (2) |
| GSE83228 |
Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA [RNA-Seq] |
| GSE83230 |
Signal-Dependent Recruitment of BRD4 to Cardiomyocyte Super-Enhancers is Suppressed by a MicroRNA |
|
| Relations |
| BioSample |
SAMN05226554 |
| SRA |
SRX1837168 |
