|
| Status |
Public on Jun 24, 2016 |
| Title |
Liver, GD13 Cylindrospermopsin, 24 hr post dose sample, 6085341028D |
| Sample type |
RNA |
| |
|
| Source name |
Liver GD13 Cylindrospermopsin, 24 hr post dose sample
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
Sex: Female
age at dosing: Gestation Day 13
chemical: Cylindrospermopsin
vehicle: Distilled water
dose: 50 ug/kg
route: IP injection
sample time: 24
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Maternal liver sampled after 1 dose on GD 13
6085341028D
|
| Data processing |
Normalized expression of probe level data was calculated in R using Bioconductor's Illumina package
|
| |
|
| Submission date |
Jun 09, 2016 |
| Last update date |
Jun 24, 2016 |
| Contact name |
Neil Chernoff |
| E-mail(s) |
chernoff.neil@epa.gov
|
| Organization name |
US EPA
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE83197 |
Adverse Health Effects of Cylindrospermopsin in Laboratory Animal (mouse) Studies in Single Day Study using Illumina Array |
| GSE83201 |
Adverse Health Effects of Cylindrospermopsin in Laboratory Animals (mouse) Studies |
|
