|
| Status |
Public on Jun 09, 2016 |
| Title |
Sertoli cells, 9d, 1 |
| Sample type |
RNA |
| |
|
| Source name |
9-day-old Sertoli cell
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: testis
|
| Treatment protocol |
After primary culture for another 24 hours, Sertoli cells were divided into 2 groups: control and 0.1mM MBP. After 24 hours culture, cells were placed on ice and treated with Trizol Reagent.
|
| Growth protocol |
Sertoli cells were isolated and cultured in DMEM/F12 medium.Testes from rats were aseptically removed, decapsulated, and digested into cell suspension. Isolated cells were placed on a tissue cultured dish in DMEM/F12 supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Genechips were scanned on the 3000 7G, an Affymetrix GeneChip scanner
|
| Description |
microRNA expression data from 9-day-old rat Sertoli cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 08, 2016 |
| Last update date |
Jun 09, 2016 |
| Contact name |
Xiaoqin Yin |
| Organization name |
School of medicine, Shanghai Jiao Tong University
|
| Department |
Shanghai Children’s Hospital
|
| Lab |
Department of endocrinology
|
| Street address |
No. 355 Luding Road, Shanghai 200062, P.R.China.
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200062 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE83106 |
Expression data from 9-day-old rat Sertoli cells and MBP-treated rat Sertoli cells |
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