|
| Status |
Public on Feb 16, 2017 |
| Title |
IGG |
| Sample type |
SRA |
| |
|
| Source name |
breast tumor
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7 from NCI-60
antibody: IgG control
|
| Treatment protocol |
CD95 stimulation was done with IgG and APO-1 100ngr/ml with protein-A 1ng/ml.
|
| Growth protocol |
Cell lines were maintained in RPMI supplemented with 10% FBS.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with anti-STAT1 antibody
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiScanSQ |
| |
|
| Description |
Chromatin IP
|
| Data processing |
Reads were aligned to the hg19 reference human genome using bwa v0.6.1
Peak-calling by HOMER v4.1
Genome_build: hg19
Genome_build: hg19
Supplementary_files_format_and_content: bedGraph file and peak txt file
|
| |
|
| Submission date |
May 25, 2016 |
| Last update date |
Sep 08, 2022 |
| Contact name |
Marcus Peter |
| E-mail(s) |
m-peter@northwestern.edu
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Street address |
303 East Superior Street, Lurie 6-123
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL15456 |
| Series (2) |
| GSE81859 |
CD95/Fas increases stemness in cancer cells by inducing a STAT1 dependent Type I interferon response [ChIP-seq] |
| GSE81860 |
CD95/Fas increases stemness in cancer cells by inducing a STAT1 dependent Type I interferon response |
|
| Relations |
| BioSample |
SAMN05173134 |
| SRA |
SRX1799016 |
