|
| Status |
Public on May 24, 2016 |
| Title |
PPI-035 BM_last |
| Sample type |
genomic |
| |
|
| Source name |
last tumor
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: myelodysplastic syndromes (MDS) patient
subject id: PPI-035
tissue type: bone marrow tumor
time point: at the end of treatment
|
| Treatment protocol |
panobinostat plus decitabine on the EOT (end of treatment) samples. Pre-study = none.
|
| Growth protocol |
none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from peripheral blood or bone marrow at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
1224826; 22938.CEL
|
| Data processing |
Copy number analysis from the SNP arrays was performed using Partek Genomics Suite (Partek, Inc),
|
| |
|
| Submission date |
May 23, 2016 |
| Last update date |
May 24, 2016 |
| Contact name |
Matt Walter |
| E-mail(s) |
mjwalter@dom.wustl.edu
|
| Organization name |
washington university in st.louis school of medicine
|
| Department |
oncology
|
| Street address |
4940 parkview
|
| City |
st.louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE81738 |
Affymetrix SNP array data for myelodysplastic syndromes samples |
|
