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Sample GSM215728 Query DataSets for GSM215728
Status Public on Aug 10, 2007
Title gibberellin treatment rep1
Sample type RNA
Source name gibberellin treated barley aleurone protoplasts
Organism Hordeum vulgare
Characteristics Protoplasts from Hordeum vulgare L. cv. Himalaya
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted from protoplasts in 1:1 (v/v) mixture of TE-saturated phenol and 0.1 M Tris-HCl buffer (pH 8.0) containing 0.1 M LiCl, 10 mM EDTA and 1 % SDS, and precipitated in 2.5 M LiCl according to Shirzadegan et al. (1991) except that extracted samples were not incubated in 80°C. Contaminated DNA was digested with DNase I (Takara, Otsu, Shiga, Japan). mRNA was purified from total RNA using Dynabeads Oligo (dT)25 (DYNAL, Dynal Biotech, Oslo, Norway) after DNase I treatment.
Label biotin
Label protocol 0.3 microgram of mRNA was processed to produce biotinylated cRNA targets.
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description Microarray analysis was performed to know how many gibberellin (GA)-responsive genes are inhibited by beta-Yariv reagent, a specific binder of plant arabinogalactan-proteins
Data processing GCOS
Submission date Aug 07, 2007
Last update date Aug 14, 2011
Contact name kiyoshi mashiguchi
Organization name University of Tokyo
Street address 1-1-1 Yayoi, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8657
Country Japan
Platform ID GPL1340
Series (1)
GSE8712 The effects of Yariv-reagent on barley aleurone GA signaling

Data table header descriptions
VALUE GCOS-calculated Signal Intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 37.574 P 0.00933744
AFFX-BioB-M_at 44.1331 P 0.000972149
AFFX-BioB-3_at 30.4175 P 0.000224668
AFFX-BioC-5_at 131.193 P 5.16732e-05
AFFX-BioC-3_at 165.926 P 7.00668e-05
AFFX-BioDn-5_at 406.567 P 4.42873e-05
AFFX-BioDn-3_at 756.315 P 4.42873e-05
AFFX-CreX-5_at 1534.95 P 4.42873e-05
AFFX-CreX-3_at 3438.67 P 4.42873e-05
AFFX-DapX-5_at 2.71512 A 0.686277
AFFX-DapX-M_at 5.4952 A 0.559354
AFFX-DapX-3_at 1.69336 A 0.834139
AFFX-LysX-5_at 3.43451 A 0.60308
AFFX-LysX-M_at 0.895491 A 0.921998
AFFX-LysX-3_at 1.6986 A 0.574038
AFFX-PheX-5_at 5.614 A 0.48511
AFFX-PheX-M_at 3.20136 A 0.824672
AFFX-PheX-3_at 7.89472 A 0.5
AFFX-ThrX-5_at 1.17645 A 0.868639
AFFX-ThrX-M_at 6.94025 A 0.313723

Total number of rows: 22840

Table truncated, full table size 794 Kbytes.

Supplementary file Size Download File type/resource
GSM215728.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM215728.CHP.gz 123.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Raw data provided as supplementary file

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