|
| Status |
Public on Jun 22, 2017 |
| Title |
X2315_nAcc_M_SL7391 |
| Sample type |
SRA |
| |
|
| Source name |
nAcc_Major Depression
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
brain region: nAcc
age at death: 58
clinical diagnosis: Major Depression
gender: M
ethnicity: Caucasian
post-mortem interval: 24
brain ph: 6.93
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Homogenates of post-mortem brain tissues were made using the MPBio FastPrep instrument, while RNAs were extracted using the Norgen Animal RNA isolation kit
RNA-seq was performed as previously described (Gertz et al. 2012)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
SL7391
|
| Data processing |
RNA-seq paired-end FASTQ files were aligned to hg19 using Tophat and Cufflinks
Read counts for each transcript were compiled across all post-mortem tissues samples
Genome_build: hg19
Supplementary_files_format_and_content: Raw read counts for all genes across all post-mortem human brain tissues (using GENCODE v9 for transcript/gene models)
|
| |
|
| Submission date |
Apr 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Bowling |
| Organization name |
HudsonAlpha Institute
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE80655 |
RNA-sequencing of human post-mortem brain tissues |
|
| Relations |
| BioSample |
SAMN04901003 |
| SRA |
SRX1725898 |
