|
| Status |
Public on Dec 19, 2017 |
| Title |
4_LPD_P21 |
| Sample type |
RNA |
| |
|
| Source name |
whole lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: P21 of life
sample origin: Low protein diet during gestation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy Qiagen kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Data processing |
The data were preprocessed and analyzing using Partek Genomic Suite.
|
| |
|
| Submission date |
Apr 11, 2016 |
| Last update date |
Dec 19, 2017 |
| Contact name |
Céline Méhats |
| E-mail(s) |
celine.mehats@inserm.fr
|
| Organization name |
Inserm
|
| Department |
U1016
|
| Street address |
24 rue du Faubourg St jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE80123 |
microRNA expression profile in whole lung of rat pups at P10 or P21 day of life after a intrauterine growth restriction due to a low protein diet during gestation |
|
