|
| Status |
Public on Jul 26, 2017 |
| Title |
lung_JF1_E18_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Total lung tissue from JF1/MsJ mice at embryonic day 18
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: JF1/MsJ
tissue: lung
developmental stage: embryonic day 18
gender: not determined
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Mice were housed in specific pathogen-free conditions. Food and water were available ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (#74104; Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
Whole Transcript (WT) Sense Target Labeling Assay (Affymetrix) without rRNA depletion.
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol.
|
| Scan protocol |
According to the Affymetrix expression protocol (Scanner 3000 7G).
|
| Description |
JF1_E18_5
Gene expression data from lung_JF1_E18_rep5.
|
| Data processing |
Expression Console (v.1.3.0.187, Affymetrix) was used to obtain normalized RMA gene-level data (standard settings including sketch-quantile normalisation).
mm9 (MGSCv37) is the genome build upon which transcript assignments are based.
'Extended' is the exon group used in the analysis.
Probe group file: MoEx-1_0-st-v1.r2.pgf
Meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.extended.mps
|
| |
|
| Submission date |
Apr 08, 2016 |
| Last update date |
Jul 26, 2017 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE80078 |
Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function |
|
