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Sample GSM210913 Query DataSets for GSM210913
Status Public on Jul 18, 2007
Title HeLa transfected with miR181a duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR181a duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR181a duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 0.030435552820563316 -0.62679147720336914 0.66319119930267334 0.22804999351501465 0.24567000567913055
10019481149 0.037322390824556351 -0.3094508945941925 0.43969550728797913 0.4724699854850769 0.51618999242782593
10019495284 -0.18922284245491028 -0.32298290729522705 0.00038294680416584015 0.59127497673034668 0.38227498531341553
10019687586 -0.025698743760585785 -1.4543256759643555 0.69208055734634399 0.036189999431371689 0.034155000001192093
10019713746 -0.015559474006295204 -1.1416850090026855 0.76536852121353149 0.075465001165866852 0.073045000433921814
10019799479 0.01373299490660429 -1.0888384580612183 0.79058247804641724 0.080310001969337463 0.082815006375312805
10019809115 0.068741090595722198 -0.23040115833282471 0.41766968369483948 0.54593503475189209 0.63964498043060303
10019874890 0.013215793296694756 -1.2573524713516235 0.78710532188415527 0.05471000075340271 0.056395001709461212
10019909307 0.21725001931190491 -1.0674176216125488 0.039892565459012985 0.067479997873306274 0.11127999424934387
10019911222 0.039143968373537064 -0.57564729452133179 0.37787142395973206 0.25402000546455383 0.27797001600265503
10019924807 -0.024617291986942291 -1.936410665512085 0.84909361600875854 0.011955000460147858 0.011345000006258488
10019927856 -0.03058573417365551 -0.23783981800079346 0.52384638786315918 0.59937000274658203 0.5583299994468689
10019932383 0.024010945111513138 -0.49277186393737793 0.62574923038482666 0.3127950131893158 0.33081001043319702
10019948931 -0.0038878796622157097 -1.5229012966156006 0.96163254976272583 0.032145000994205475 0.031704999506473541
10019975533 -0.018783263862133026 -2.0783343315124512 0.92689770460128784 0.0091850003227591515 0.009089999832212925
10019977227 0.03939405083656311 -0.59323501586914063 0.713642418384552 0.24679499864578247 0.26942998170852661
10019987588 0.016334971413016319 -1.0319092273712158 0.7544366717338562 0.091189995408058167 0.094775006175041199
10020008603 -0.0017168794292956591 -0.91711831092834473 0.97360384464263916 0.12533499300479889 0.12406999617815018
10020014121 -0.05404939129948616 -0.09535081684589386 0.54368859529495239 0.86627000570297241 0.75374001264572144
10020044367 0.012084215879440308 -0.95193350315093994 0.84178072214126587 0.1111999973654747 0.11561000347137451

Total number of rows: 23652

Table truncated, full table size 2636 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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