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Sample GSM210911 Query DataSets for GSM210911
Status Public on Jul 18, 2007
Title HeLa transfected with miR148b duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR148b duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR148b duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 0.36716723442077637 -0.69862651824951172 3.9260692119569285e-007 0.13120999932289124 0.30618500709533691
10019481149 -0.064298294484615326 -0.32727319002151489 0.17440220713615417 0.50700497627258301 0.43710500001907349
10019495284 0.03493320569396019 -0.07856316864490509 0.47720563411712646 0.80439496040344238 0.87027502059936523
10019687586 0.0004168548621237278 -1.2321536540985107 0.99545556306838989 0.059839997440576553 0.059725001454353333
10019713746 0.050471082329750061 -0.41662850975990295 0.5327599048614502 0.36347499489784241 0.42245501279830933
10019799479 0.031035000458359718 -0.79566609859466553 0.6365506649017334 0.15462499856948853 0.1661050021648407
10019809115 0.3257160484790802 -0.38551679253578186 4.3710853788070381e-005 0.28477001190185547 0.60159498453140259
10019874890 0.024454342201352119 -1.0049211978912354 0.63208025693893433 0.096730001270771027 0.10240499675273895
10019909307 0.30610406398773193 -0.63735592365264893 3.290312088211067e-005 0.16595999896526337 0.33154499530792236
10019911222 0.079783067107200623 -0.17556679248809814 0.19693319499492645 0.61003494262695313 0.73209500312805176
10019924807 0.01380655262619257 -1.7116184234619141 0.90653949975967407 0.01919499970972538 0.019689999520778656
10019927856 -0.077223233878612518 -0.10544925928115845 0.11604197323322296 0.85776495933532715 0.71865999698638916
10019932383 -0.006409776397049427 -0.35800546407699585 0.91816741228103638 0.44220000505447388 0.43568497896194458
10019948931 0.034973625093698502 -1.4077756404876709 0.70720213651657104 0.039664998650550842 0.043059997260570526
10019975533 0.066100642085075378 -1.9981414079666138 0.76164650917053223 0.013839999213814735 0.014884999953210354
10019977227 -0.077495403587818146 -0.18980374932289124 0.12175124883651733 0.70645999908447266 0.5907900333404541
10019987588 0.016893846914172173 -0.63941597938537598 0.73858010768890381 0.2259100079536438 0.23551499843597412
10020008603 0.027381874620914459 -0.63941746950149536 0.65506750345230103 0.22306500375270844 0.23679500818252563
10020014121 -0.0031321803107857704 -0.052348744124174118 0.94927418231964111 0.89708501100540161 0.89394998550415039
10020044367 -0.0059967045672237873 -0.95502865314483643 0.91349929571151733 0.1229500025510788 0.12166000157594681

Total number of rows: 23578

Table truncated, full table size 2611 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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