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Sample GSM210907 Query DataSets for GSM210907
Status Public on Jul 18, 2007
Title HeLa transfected with miR133a duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR133a duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR133a duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 2.96E-02 -9.80E-01 6.55E-01 1.02E-01 1.09E-01
10019481149 -5.76E-02 -3.68E-01 2.85E-01 4.60E-01 4.01E-01
10019495284 7.43E-02 -2.33E-01 1.31E-01 5.38E-01 6.38E-01
10019687586 -1.09E-02 -1.455 9.20E-01 3.55E-02 3.50E-02
10019713746 -1.53E-02 -1.192 8.12E-01 6.84E-02 6.65E-02
10019799479 -4.85E-05 -1.013 9.99E-01 9.71E-02 9.72E-02
10019809115 2.57E-02 -8.96E-01 6.36E-01 1.25E-01 1.32E-01
10019874890 1.20E-01 -9.67E-01 5.02E-02 9.56E-02 1.25E-01
10019909307 9.10E-02 -9.08E-01 1.82E-01 1.11E-01 1.38E-01
10019911222 4.40E-02 -7.32E-01 4.26E-01 1.77E-01 1.95E-01
10019924807 3.23E-03 -1.781 9.86E-01 1.70E-02 1.72E-02
10019927856 -4.08E-02 -3.20E-01 4.46E-01 5.02E-01 4.57E-01
10019932383 6.51E-03 -3.92E-01 9.11E-01 4.03E-01 4.09E-01
10019948931 9.08E-03 -1.201 9.01E-01 6.28E-02 6.50E-02
10019975533 -3.32E-02 -1.609 8.22E-01 2.51E-02 2.44E-02
10019977227 -2.08E-01 -6.92E-01 2.09E-02 2.60E-01 1.60E-01
10019987588 1.84E-02 -1.093 7.42E-01 7.92E-02 8.26E-02
10020008603 1.48E-02 -8.58E-01 8.20E-01 1.37E-01 1.41E-01
10020014121 1.22E-01 -1.26E-01 1.95E-01 6.52E-01 8.68E-01
10020044367 1.65E-02 -7.80E-01 7.61E-01 1.63E-01 1.70E-01

Total number of rows: 23653

Table truncated, full table size 1278 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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