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Sample GSM210904 Query DataSets for GSM210904
Status Public on Jul 18, 2007
Title HeLa transfected with miR132 duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR132 duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR132 duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 0.10046140849590302 -0.61673057079315186 0.15757845342159271 0.21784999966621399 0.27200999855995178
10019481149 0.071450605988502502 -0.30786675214767456 0.13871189951896667 0.45425999164581299 0.53633499145507813
10019495284 -0.0018739310326054692 -0.30274271965026855 0.96703493595123291 0.5027649998664856 0.50011998414993286
10019687586 -0.025125836953520775 -1.5237728357315063 0.70012509822845459 0.031040001660585403 0.029295001178979874
10019713746 -5.7455694332020357e-005 -1.1486622095108032 0.99930423498153687 0.072044998407363892 0.071445003151893616
10019799479 0.019759485498070717 -1.0173790454864502 0.68519020080566406 0.097534999251365662 0.10188499838113785
10019809115 0.14418870210647583 -0.26453018188476563 0.10547807812690735 0.45973998308181763 0.64960002899169922
10019874890 0.0002978156553581357 -1.3213107585906982 0.99620318412780762 0.047880001366138458 0.047759998589754105
10019909307 0.1765950471162796 -1.0589761734008789 0.053769230842590332 0.071429997682571411 0.1078449934720993
10019911222 0.055839706212282181 -0.5638049840927124 0.20369671285152435 0.2562900185585022 0.29145500063896179
10019924807 -0.034565944224596024 -1.8647127151489258 0.75518906116485596 0.014220000244677067 0.013214999809861183
10019927856 -0.014053808525204659 -0.22012187540531158 0.77830857038497925 0.61228001117706299 0.59279000759124756
10019932383 0.072000347077846527 -0.47963136434555054 0.12446363270282745 0.30505499243736267 0.36015498638153076
10019948931 0.0038662946317344904 -1.5639374256134033 0.96288782358169556 0.027289999648928642 0.027589999139308929
10019975533 0.0053433259017765522 -2.037285327911377 0.96941953897476196 0.009154999628663063 0.0092500001192092896
10019977227 -0.063062265515327454 -0.607322096824646 0.54845678806304932 0.26877999305725098 0.23125000298023224
10019987588 0.02150999940931797 -1.2371922731399536 0.87265801429748535 0.056499999016523361 0.059369999915361404
10020008603 0.026441249996423721 -0.92896711826324463 0.63619816303253174 0.11682000756263733 0.12538999319076538
10020014121 -0.049362514168024063 -0.06463979184627533 0.58942675590515137 0.9196549654006958 0.81674498319625854
10020044367 -0.030573166906833649 -0.98573994636535645 0.60023397207260132 0.10728500038385391 0.10042499750852585

Total number of rows: 23653

Table truncated, full table size 2635 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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