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Sample GSM210898 Query DataSets for GSM210898
Status Public on Jul 18, 2007
Title HeLa transfected with miR9 duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR9 duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR9 duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 0.067394904792308807 -0.90964305400848389 0.35404872894287109 0.11415000259876251 0.13365499675273895
10019481149 -0.010124442167580128 -0.34232541918754578 0.85330772399902344 0.46006500720977783 0.44957000017166138
10019495284 -0.020712269470095634 0.092057883739471436 0.69532924890518188 1.271090030670166 1.2082450389862061
10019687586 0.053226161748170853 -1.2603218555450439 0.48867738246917725 0.051820002496242523 0.058240000158548355
10019713746 -0.02666052058339119 -1.0142292976379395 0.64644002914428711 0.10066500306129456 0.094685003161430359
10019799479 -0.012086397968232632 -1.0532562732696533 0.83678704500198364 0.090149998664855957 0.087664999067783356
10019809115 0.066159568727016449 -0.45619869232177734 0.42117011547088623 0.32569998502731323 0.37888497114181519
10019874890 0.0036377974320203066 -1.0254940986633301 0.94795584678649902 0.093940004706382751 0.094659999012947083
10019909307 -0.025847939774394035 -1.2307567596435547 0.76317638158798218 0.060134999454021454 0.057640001177787781
10019911222 -0.028880665078759193 -0.20649805665016174 0.51065915822982788 0.6442750096321106 0.60286498069763184
10019924807 0.02519448846578598 -1.9768447875976563 0.92812138795852661 0.010084999725222588 0.011265000328421593
10019927856 -0.1233404278755188 0.10767483711242676 0.071820221841335297 1.4788500070571899 1.1155600547790527
10019932383 0.028912758454680443 -0.49048471450805664 0.63437598943710327 0.31507998704910278 0.33451500535011292
10019948931 0.048163097351789474 -1.4048190116882324 0.6401713490486145 0.038139998912811279 0.042229998856782913
10019975533 -0.013814507983624935 -1.7744436264038086 0.94403076171875 0.017514999955892563 0.017839999869465828
10019977227 -0.065892726182937622 -0.4056549072265625 0.35778307914733887 0.42421501874923706 0.36726999282836914
10019987588 -0.0017744206124916673 -0.96570301055908203 0.97634756565093994 0.10879500210285187 0.10825499892234802
10020008603 -0.0068141394294798374 -0.95399463176727295 0.92943763732910156 0.11510500311851501 0.11410500109195709
10020014121 0.056939296424388885 -0.1858518123626709 0.42306509613990784 0.61116999387741089 0.6978449821472168
10020044367 -0.022896915674209595 -0.68097412586212158 0.65496826171875 0.21410000324249268 0.20304000377655029

Total number of rows: 23651

Table truncated, full table size 2630 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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