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Sample GSM210897 Query DataSets for GSM210897
Status Public on Jul 18, 2007
Title HeLa transfected with miR7 duplex versus control transfected HeLa, 24 hours
Sample type RNA
 
Channel 1
Source name Mock transfected HeLa, 24 hours
Organism Homo sapiens
Characteristics Mock transfected HeLa, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy3
Label protocol Customized Ambion MessageAmp II
 
Channel 2
Source name HeLa transfected with miR7 duplex, 24 hours
Organism Homo sapiens
Characteristics HeLa transfected with miR7 duplex, 24 hours
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label Cy5
Label protocol Customized Ambion MessageAmp II
 
 
Hybridization protocol Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7.
Scan protocol Agilent LP2 laser scanner
Description Transfection of miRNA duplexes into cell lines as described in Grimson et al., Molecular Cell, 2007
Data processing Error model as described in Hughes et al and in US patent #6,351,712
 
Submission date Jul 16, 2007
Last update date Jul 18, 2007
Contact name Lee Lim
E-mail(s) lee_lim@merck.com
Phone 206-802-7323
Organization name Rosetta Inpharmatics
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3991
Series (1)
GSE8501 MicroRNA targeting specificity in mammals: determinants beyond seed pairing

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted]
LOG_INTENSITY Averaged log10 intensity of a fluor-reversed chip pair
P_VALUE P value that the probe target is differentially expressed in the two tissues
INTENSITY1 CHANNEL1 intensity; averaged raw data from a fluor-reversed pair
INTENSITY2 CHANNEL2 intensity; averaged raw data from a fluor-reversed pair

Data table
ID_REF VALUE LOG_INTENSITY P_VALUE INTENSITY1 INTENSITY2
10019475365 -0.18108588457107544 -0.80372834205627441 0.0057944860309362411 0.19440999627113342 0.12742999196052551
10019481149 -0.011940499767661095 -0.34785118699073792 0.79138630628585815 0.45680499076843262 0.44414001703262329
10019495284 0.0097195003181695938 -0.19350774586200714 0.83257567882537842 0.63333499431610107 0.64769500494003296
10019687586 0.0093497131019830704 -1.503071665763855 0.87057536840438843 0.031179998070001602 0.031840000301599503
10019713746 -0.03404606506228447 -1.153658390045166 0.51156997680664063 0.077305004000663757 0.071615003049373627
10019799479 -0.020799936726689339 -1.2609826326370239 0.73219335079193115 0.056170001626014709 0.05356999859213829
10019809115 -0.15105549991130829 -0.39000394940376282 0.048758525401353836 0.49465000629425049 0.34186500310897827
10019874890 -0.018754497170448303 -1.2821767330169678 0.7098691463470459 0.053360000252723694 0.051114998757839203
10019909307 0.0085262581706047058 -1.1096439361572266 0.90015041828155518 0.076995000243186951 0.079154998064041138
10019911222 -0.037071079015731812 -0.6209266185760498 0.46906188130378723 0.2498449981212616 0.2294050008058548
10019924807 0.010145164094865322 -2.0334913730621338 0.95079934597015381 0.0091500002890825272 0.0095249991863965988
10019927856 -0.010750249959528446 -0.22211045026779175 0.82474452257156372 0.60747003555297852 0.59229499101638794
10019932383 0.0084060002118349075 -0.4540073573589325 0.87070286273956299 0.34839999675750732 0.35497498512268066
10019948931 0.022549349814653397 -1.503976583480835 0.76473897695541382 0.030714999884366989 0.032540000975131989
10019975533 0.031469900161027908 -1.8890810012817383 0.81273359060287476 0.012900000438094139 0.014045000076293945
10019977227 0.038728799670934677 -0.5402604341506958 0.68365347385406494 0.27623501420021057 0.30493497848510742
10019987588 -0.026642261072993279 -1.2139099836349487 0.6419340968132019 0.063539996743202209 0.05950000137090683
10020008603 -0.011455785483121872 -1.1402595043182373 0.83490478992462158 0.073389999568462372 0.071534998714923859
10020014121 -0.032344549894332886 -0.074412062764167786 0.70512574911117554 0.87849998474121094 0.81543999910354614
10020044367 -0.029059005901217461 -0.95805227756500244 0.58961528539657593 0.11477500200271606 0.10688500106334686

Total number of rows: 23653

Table truncated, full table size 2637 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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