|
| Status |
Public on Jul 11, 2007 |
| Title |
pbx2-MO;pbx4-MO, 18s, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish 18 somite stage embryos
|
| Organism |
Danio rerio |
| Characteristics |
AB background, 18 somite embryos
|
| Treatment protocol |
Embryos were injected at the 1-2 cell stage with pbx2 and pbx4 morpholinos
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
|
| |
|
| Hybridization protocol |
cRNA was hybridized onto Affymetrix zebrafish genome arrays
|
| Scan protocol |
GeneChips were scanned with GENECHIP software
|
| Description |
Gene expression data from zebrafish 18 somite stage embryos
|
| Data processing |
The data were normalized by RMA using the Affy package of Bioconductor
|
| |
|
| Submission date |
Jul 10, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Yi Cao |
| E-mail(s) |
ycao@fhcrc.org
|
| Phone |
206-667-5278
|
| Organization name |
Fred Hutchinson CRC
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview Ave. N
|
| City |
Seatttle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE8428 |
The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites. |
|
