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Sample GSM2071654 Query DataSets for GSM2071654
Status Public on Mar 18, 2016
Title total RNA-seq from lung (ENCLB626WMA)
Sample type SRA
 
Source name Mus musculus strain B6NCrl lung tissue mixed sex embryo (16.5 days)
Organism Mus musculus
Characteristics tissue_type: lung
biomaterial_type: tissue
description: Lung from 41 embyonic 16.5 day mice
biosample encode accession: ENCBS833ZFI (SAMN04284156)
age: 16.5 day
donor_id: ENCDO956IXV
dev stage: embryonic
Sex: pooled male and female
health state: healthy
lab: Len Pennacchio, LBNL
link: ENCBS833ZFI at ENCODE; https://www.encodeproject.org/ENCBS833ZFI/
link: Derived from ENCODE donor ENCDO956IXV; https://www.encodeproject.org/ENCDO956IXV/
link: excision protocol; https://www.encodeproject.org/documents/631aa21c-8e48-467e-8cac-d40c875b3913/@@download/attachment/StandardTissueExcisionProtocol_02132017.pdf
Extracted molecule total RNA
Extraction protocol extraction method: Ambion mirVana
extraction protocol: https://www.encodeproject.org/documents/428a184d-7fa1-4599-9d8d-749c2eba7edd/@@download/attachment/cms_055740.pdf, extraction protocol: https://www.encodeproject.org/documents/49fd5b3b-9878-4ec8-92bf-ef6dce9116e3/@@download/attachment/SMARTer%20Ultra%20Low%20RNA%20Kit%20for%20Illumina%20Sequencing%20User%20Manual%20%28PT5163-1%29_120213.pdf, extraction protocol: https://www.encodeproject.org/documents/f0cc5a7f-96a5-4970-9f46-317cc8e2d6a4/@@download/attachment/cms_055423.pdf
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description https://www.encodeproject.org/experiments/ENCSR992WBR/
***************
biological replicate number: 2
technical replicate number: 1
description: RNA-Seq on embryonic 16.5 day mouse lung
experiment encode accession: ENCSR992WBR
assay title: total RNA-seq
assembly: mm10
encode release date: 2015-10-13
lab: Barbara Wold, Caltech
library encode accession: ENCLB626WMA
size range: >200
spikeins used: /references/ENCSR884LPM/
nucleic acid starting quantity: 10
nucleic acid starting quantity units: ng
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Feb 24, 2016
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL17021
Series (1)
GSE78496 total RNA-seq from lung (ENCSR992WBR)
Relations
SRA SRX1603334
BioSample SAMN04284156
Named Annotation GSM2071654_ENCFF295ZRQ_signal_of_unique_reads_mm10.bigWig
Named Annotation GSM2071654_ENCFF767MKY_signal_of_all_reads_mm10.bigWig

Supplementary file Size Download File type/resource
GSM2071654_ENCFF295ZRQ_signal_of_unique_reads_mm10.bigWig 132.4 Mb (ftp)(http) BIGWIG
GSM2071654_ENCFF365SND_gene_quantifications_mm10.tsv.gz 2.1 Mb (ftp)(http) TSV
GSM2071654_ENCFF740VFI_transcript_quantifications_mm10.tsv.gz 4.5 Mb (ftp)(http) TSV
GSM2071654_ENCFF767MKY_signal_of_all_reads_mm10.bigWig 155.5 Mb (ftp)(http) BIGWIG
GSM2071654_ENCLB626WMA_README.txt.gz 646 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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