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GEO accession:
Sample GSM2071528
Query DataSets for GSM2071528
Status
Public on Mar 18, 2016
Title
total RNA-seq from midbrain (ENCLB080NNG)
Sample type
SRA
Source name
Mus musculus strain B6NCrl midbrain tissue postnatal (0 days)
Organism
Mus musculus
Characteristics
tissue_type: midbrain
biomaterial_type: tissue
description: single midbrain from pup 1
biosample encode accession: ENCBS169PZD (SAMN04284019)
age: 0 day
donor_id: ENCDO956IXV
dev stage: postnatal
Sex: not collected
lab: Barbara Wold, Caltech
link: ENCBS169PZD at ENCODE;
https://www.encodeproject.org/ENCBS169PZD/
link: Derived from ENCODE donor ENCDO956IXV;
https://www.encodeproject.org/ENCDO956IXV/
Extracted molecule
total RNA
Extraction protocol
extraction method: Ambion mirVana
extraction protocol:
https://www.encodeproject.org/documents/428a184d-7fa1-4599-9d8d-749c2eba7edd/@@download/attachment/cms_055740.pdf,
extraction protocol:
https://www.encodeproject.org/documents/49fd5b3b-9878-4ec8-92bf-ef6dce9116e3/@@download/attachment/SMARTer%20Ultra%20Low%20RNA%20Kit%20for%20Illumina%20Sequencing%20User%20Manual%20%28PT5163-1%29_120213.pdf,
extraction protocol:
https://www.encodeproject.org/documents/f0cc5a7f-96a5-4970-9f46-317cc8e2d6a4/@@download/attachment/cms_055423.pdf
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
cDNA
Instrument model
Illumina HiSeq 2500
Description
https://www.encodeproject.org/experiments/ENCSR719NAJ/
***************
biological replicate number: 1
technical replicate number: 1
description: Total RNA-Seq on postnatal 0 day mouse midbrain
experiment encode accession: ENCSR719NAJ
assay title: total RNA-seq
assembly: mm10
encode release date: 2015-06-23
lab: Barbara Wold, Caltech
library encode accession: ENCLB080NNG
size range: >200
spikeins used: /references/ENCSR013YHQ/, /references/ENCSR133ALU/
nucleic acid starting quantity: 10.0
nucleic acid starting quantity units: ng
Data processing
See GSM*_README.txt supplementary file linked below
Submission date
Feb 24, 2016
Last update date
May 15, 2019
Contact name
ENCODE DCC
E-mail(s)
encode-help@lists.stanford.edu
Organization name
ENCODE DCC
Street address
300 Pasteur Dr
City
Stanford
State/province
CA
ZIP/Postal code
94305-5120
Country
USA
Platform ID
GPL17021
Series (1)
GSE78437
total RNA-seq from midbrain (ENCSR719NAJ)
Relations
SRA
SRX1603219
BioSample
SAMN04284019
Named Annotation
GSM2071528_ENCFF398USF_signal_of_unique_reads_mm10.bigWig
Named Annotation
GSM2071528_ENCFF964YBJ_signal_of_all_reads_mm10.bigWig
Supplementary file
Size
Download
File type/resource
GSM2071528_ENCFF210MWH_gene_quantifications_mm10.tsv.gz
2.1 Mb
(ftp)
(http)
TSV
GSM2071528_ENCFF398USF_signal_of_unique_reads_mm10.bigWig
134.9 Mb
(ftp)
(http)
BIGWIG
GSM2071528_ENCFF484PSX_transcript_quantifications_mm10.tsv.gz
4.5 Mb
(ftp)
(http)
TSV
GSM2071528_ENCFF964YBJ_signal_of_all_reads_mm10.bigWig
154.3 Mb
(ftp)
(http)
BIGWIG
GSM2071528_ENCLB080NNG_README.txt.gz
643 b
(ftp)
(http)
TXT
SRA Run Selector
Raw data are available in SRA
Processed data provided as supplementary file
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