|
| Status |
Public on Feb 24, 2016 |
| Title |
VdG30 |
| Sample type |
RNA |
| |
|
| Source name |
CWR-R1, Parental Untreated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CWR-R1
tissue: Prostate Cancer Cell Line
|
| Treatment protocol |
Cells were treated with vehicle (DMSO), or 10uM enzalutamide and RNA extracted directly from plate.
|
| Growth protocol |
Cells grown in their respective growth media with 10% FBS and Pen/Strep. Cells were treated either with DMSO vehicle (Parental Untreated); 10uM MDV3100/Enzalutamide for 48 hours (48hrs MDV3100), or for at least 6 months in 10uM MDV3100 (MDV3100-Resistant)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
idat file: 9257302030
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Feb 23, 2016 |
| Last update date |
Feb 24, 2016 |
| Contact name |
Donald Vander Griend |
| E-mail(s) |
dvanderg@uic.edu
|
| Organization name |
The University of Illinois at Chicago
|
| Department |
Pathology
|
| Lab |
Vander Griend
|
| Street address |
909 S. Wolcott Ave, COMRB Room 5051
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE78201 |
Acquired Resistance to the Second-Generation Androgen Receptor Antagonist Enzalutamide in Castration-Resistant Prostate Cancer |
|
