|
| Status |
Public on Feb 16, 2016 |
| Title |
Human Peripheral Blood monocytes [Sample_52] |
| Sample type |
RNA |
| |
|
| Source name |
aPD1+aCTLA4-post sequential therapy sample (F2)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
individual: F
agent: Seq
time point: post sequential therapy sample
cell type: Peripheral Blood monocytes
|
| Growth protocol |
Not Applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
|
| |
|
| Submission date |
Feb 15, 2016 |
| Last update date |
Feb 16, 2016 |
| Contact name |
Rakesh Verma |
| E-mail(s) |
rakesh.verma@yale.edu
|
| Organization name |
Yale University
|
| Department |
Yale Cancer Center
|
| Lab |
Hematology
|
| Street address |
333 Cedar St.
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platform ID |
GPL17585 |
| Series (1) |
| GSE77924 |
Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo |
|
