|
| Status |
Public on Feb 16, 2016 |
| Title |
Mock Array33_Cy3 |
| Sample type |
RNA |
| |
|
| Source name |
L4
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: control
developmental stage: L4
|
| Treatment protocol |
NA
|
| Growth protocol |
Synchronized developmental stage L4 worms, grown as previously described Brenner 1974 on nematode growth medium (NGM) agar plates seeded with Escherichia coli strain OP50 at 20 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was done using RNEasy Micro Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Two-Color Microarray-Based Gene Expression Analysis’-protocol from Agilent
|
| |
|
| Hybridization protocol |
Two-Color Microarray-Based Gene Expression Analysis’-protocol from Agilent
|
| Scan protocol |
Agilent Feature Extraction Software
|
| Data processing |
For normalization the Limma package was used with for Agilent array recommended settings. No background correction was performed. For within-array normalization, the LOESS method was used and for between-array normalization, the quantile method was used
|
| |
|
| Submission date |
Feb 13, 2016 |
| Last update date |
Feb 16, 2016 |
| Contact name |
Basten Snoek |
| E-mail(s) |
snoek.basten@gmail.com
|
| Organization name |
Utrecht University
|
| Department |
Mol. Plant. Physiology
|
| Lab |
van Zanten
|
| Street address |
Padualaan
|
| City |
Utrecht |
| ZIP/Postal code |
3584CH |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10094 |
| Series (1) |
| GSE77905 |
Natural Genetic Variation Influences Protein Abundances in C. elegans Developmental Signalling Pathways |
|
