|
| Status |
Public on Feb 10, 2016 |
| Title |
Human Peripheral Blood T cells [Sample_34] |
| Sample type |
RNA |
| |
|
| Source name |
Human Peripheral Blood T cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: post
|
| Growth protocol |
Not Applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
aPD1+aCTLA4-post
|
| Data processing |
Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
|
| |
|
| Submission date |
Feb 09, 2016 |
| Last update date |
Feb 10, 2016 |
| Contact name |
Rakesh Verma |
| E-mail(s) |
rakesh.verma@yale.edu
|
| Organization name |
Yale University
|
| Department |
Yale Cancer Center
|
| Lab |
Hematology
|
| Street address |
333 Cedar St.
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platform ID |
GPL17585 |
| Series (1) |
| GSE77714 |
Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo |
|
