|
Status |
Public on Dec 06, 2016 |
Title |
251 |
Sample type |
SRA |
|
|
Source name |
Differentiated mouse embryonic stem cells
|
Organism |
Mus musculus |
Characteristics |
reporter construct: -8/P/+7/+9 FLK1+/LacZ+ r3
|
Extracted molecule |
total RNA |
Extraction protocol |
All RNA was extracted from sorted ES cells using RNA extraction kit and amplified using Nugen RNA amplification kit Library construction was performed as per Illumina protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Reads were mapped to mouse genome (UCSC, mm10) using STAR (v2.4.0). The resulting sam files were sorted and HTSeq (v0.6.1) was used to generate counts (with options --mode=intersection-nonempty --stranded=no). Genome_build: mm10 Supplementary_files_format_and_content: txt with raw read counts
|
|
|
Submission date |
Jan 29, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Dominik Beck |
E-mail(s) |
dominik.beck@uts.edu.au
|
Organization name |
University of Technology Sydney
|
Department |
School of Biomedical Engineering
|
Street address |
Level 10, UTS Building 11 (CB11 81-113, Broadway, Ultimo)
|
City |
Sydney |
State/province |
NSW |
ZIP/Postal code |
2007 |
Country |
Australia |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE77390 |
Probing Hemogenic Precursors with Endoglin Regulatory Elements uncovers LRP2 as a Regulator of Hematopoiesis |
|
Relations |
BioSample |
SAMN04448258 |
SRA |
SRX1553590 |