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Sample GSM2051239 Query DataSets for GSM2051239
Status Public on Dec 06, 2016
Title 251
Sample type SRA
 
Source name Differentiated mouse embryonic stem cells
Organism Mus musculus
Characteristics reporter construct: -8/P/+7/+9 FLK1+/LacZ+ r3
Extracted molecule total RNA
Extraction protocol All RNA was extracted from sorted ES cells using RNA extraction kit and amplified using Nugen RNA amplification kit
Library construction was performed as per Illumina protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Reads were mapped to mouse genome (UCSC, mm10) using STAR (v2.4.0).
The resulting sam files were sorted and HTSeq (v0.6.1) was used to generate counts (with options --mode=intersection-nonempty --stranded=no).
Genome_build: mm10
Supplementary_files_format_and_content: txt with raw read counts
 
Submission date Jan 29, 2016
Last update date May 15, 2019
Contact name Dominik Beck
E-mail(s) dominik.beck@uts.edu.au
Organization name University of Technology Sydney
Department School of Biomedical Engineering
Street address Level 10, UTS Building 11 (CB11 81-113, Broadway, Ultimo)
City Sydney
State/province NSW
ZIP/Postal code 2007
Country Australia
 
Platform ID GPL13112
Series (1)
GSE77390 Probing Hemogenic Precursors with Endoglin Regulatory Elements uncovers LRP2 as a Regulator of Hematopoiesis
Relations
BioSample SAMN04448258
SRA SRX1553590

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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