|
| Status |
Public on Feb 25, 2017 |
| Title |
wt deleted cells |
| Sample type |
SRA |
| |
|
| Source name |
wt deleted cells
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
growth stage: log growing cells
genotype/variation: Wild type
|
| Treatment protocol |
Genomic deletion of ago1d, mmi1, and dcr1.
|
| Growth protocol |
Standard conditions were used to produce logarithmically growing cultures in rich media (YEA). Cultures were grown at 30C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the MasterPure™ Yeast RNA Purification Kit (epicentre)
ScriptSeq v2 RNA-Seq Library Preparation Kit was used according to manufacturer's recommendation.
Libraries were analyzed using an Agilent 2100 BioAnalyzer (Agilent) and sequenced on the Illumina MiSeq platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Alignment was done using Tophat.
FPKM values were calculated using cufflinks
Genome_build: asm294v2.28
Supplementary_files_format_and_content: Tab delimited text files, from cufflinks
|
| |
|
| Submission date |
Jan 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Shiv Grewal |
| Phone |
2407607553
|
| Organization name |
NCI
|
| Department |
LBMB
|
| Lab |
Shiv Grewal
|
| Street address |
NCI bldg 37 Rm 6068 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL16192 |
| Series (2) |
| GSE77047 |
Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy (RNA-seq) |
| GSE77050 |
Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy |
|
| Relations |
| BioSample |
SAMN04431485 |
| SRA |
SRX1539180 |
