|Public on Jun 21, 2007
|B73xMo17 immature ear tissue, biological replicate 1
|Zea mays immature ear tissue, genotype B73xMo17
|Stage: immature ear (3.7-5.4mm); Genotype: B73xMo17
|Immature ears from inbreds Mo17 and B73
and hybrids Mo17xB73 and B73xMo17 were collected from
field-grown maize plants during July 2005 for gene expression
analysis. The maize plants were grown on the St. Paul campus
Agricultural Experiment Station. Ears were collected for each
genotype between 9 am and 11 am on each of three consecutive
days from various positions in the field. Ears ranging
from 3.7 to 5.4 mm in length were sampled. Ears were flash
frozen in liquid nitrogen following the manual removal of
husks and silks.
|For immature ear RNA isolation, tissues from 6 ears/genotype/biological replicate were pooled and ground in liquid nitrogen. RNAs were extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). All RNAs were subsequently subjected to DNAse treatment and phenol:chloroform extraction. RNAs were precipitated with 0.13 volume sodium acetate (pH 5.5) and 2.53 volume ethanol. Resuspended RNAs were purified further using the RNeasy system, according to the manufacturer’s instructions (QIAGEN, Valencia, CA).
|Eight ug of total RNA was labeled for each hybridization using the One-Cycle cDNA Synthesis Kit, according to the manufacturer’s instructions (Affymetrix, Santa Clara CA).
|Hybridization was performed according to the recommended Affymetrix protocols at the University of Minnesota Microarray facility.
|The Genechip 3000 scanner was used to scan each array
|refer to PMID: 16702414
|MAS5.0 values are reported; the .cel file is also available
|Jun 19, 2007
|Last update date
|Aug 14, 2011
|Nathan M Springer
|University of Minnesota
|1445 Gortner Ave
|Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Immature ear data
|Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression