|
| Status |
Public on Dec 31, 2015 |
| Title |
Adipocytes_Dhet.mice_Chow.diet_16weeks, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Adipocytes obtained from Dhet mice fed Chow diet for 16 weeks
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
gender: Female
genotype: IR+/- IRS1+/- ApoE-/-
tissue: parametrial and periovarian adipose tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adipocytes were isolated from parametrial and periovarian adipose tissues and total RNA was extracted from the adipocytes using TRIzol (Life Technologies Corporation).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol starting with 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array (Affymetrix MG430-2.0 GeneChips). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
|
| Description |
Gene.Expression.Data from adipose tissue of mice fed chow diet
|
| Data processing |
The raw intensity values of hybridization were scanned and stored as .CEL files using Affymetrix Microarray suite software with global scaling option. Gene expression values were obtained by reading and normalizing the .CEL files with Robust Multi Average (RMA) express software using the cdf file Mouse430_2.cdf
|
| |
|
| Submission date |
Dec 30, 2015 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Annamalai Muthiah |
| E-mail(s) |
am2ta@virginia.edu
|
| Phone |
5404420248
|
| Organization name |
University of Virginia
|
| Street address |
857, Swan Ridge Road
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE76428 |
Gene expression data from adipocytes of insulin resistant mice |
|
