|
Status |
Public on Jan 05, 2008 |
Title |
Control sample, Sample_ID = 123a, DNA |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
genomic DNA from ASO1
|
Organism |
Homo sapiens |
Characteristics |
CEPH family: CEPH 1421 CEPH pedigree: MOTHER
|
Extracted molecule |
genomic DNA |
Extraction protocol |
RNA was extracted from four to five 5-um sections using an RNA extraction kit (Roche High Pure RNA Paraffin kit), yielding 0.5 - 3 ug of total RNA.
|
Label |
Cy3
|
Label protocol |
GoldenGateĀ® assay was done as described previously (Fan JB et al, Illumina Universal bead arrays, 2006)
|
|
|
Channel 2 |
Source name |
genomic DNA from ASO2
|
Organism |
Homo sapiens |
Characteristics |
CEPH family: CEPH 1421 CEPH pedigree: MOTHER
|
Extracted molecule |
genomic DNA |
Extraction protocol |
RNA was extracted from four to five 5-um sections using an RNA extraction kit (Roche High Pure RNA Paraffin kit), yielding 0.5 - 3 ug of total RNA.
|
Label |
Cy5
|
Label protocol |
GoldenGateĀ® assay was done as described previously (Fan JB et al, Illumina Universal bead arrays, 2006)
|
|
|
|
Hybridization protocol |
Array image processing and signal extraction were done as described previously (Fan JB et al, Illumina Universal bead arrays, 2006)
|
Scan protocol |
Arrays were imaged using Illumina BeadArray Reader scanner (Illumina).
|
Description |
none
|
Data processing |
Image analysis and data extraction software were as described previously (Oliphant, A., Barker, D.L., Stuelpnagel, J.R., and Chee, M.S. 2002. BeadArray technology: enabling an accurate, cost-effective approach to high-throughput genotyping. Biotechniques 32: S56-S61.; Galinsky, V.L. 2003. Automatic registration of microarray images II: Hexagonal grid. Bioinformatics 19: 1832-1836.). Each sequence type is represented by an average of thirty beads on the array. Bead signals are computed with weighted averages of pixel intensities, and local background is subtracted. Sequence-type signal is calculated by averaging corresponding bead signals with outliers removed (using median absolute deviation).
|
|
|
Submission date |
Jun 08, 2007 |
Last update date |
Nov 13, 2007 |
Contact name |
Aik Choon Tan |
E-mail(s) |
actan@jhu.edu
|
Phone |
410-502-8743
|
Fax |
410-614-9006
|
Organization name |
Johns Hopkins University
|
Department |
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins
|
Street address |
1650 Orleans Street, RM 1M86
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21231 |
Country |
USA |
|
|
Platform ID |
GPL5358 |
Series (2) |
GSE8054 |
Allele-Specific Expression 1 |
GSE9596 |
Allele-specific expression in familial pancreatic cancer |
|