|
| Status |
Public on Dec 29, 2020 |
| Title |
CL1-0 cells expressing PSAT1 |
| Sample type |
RNA |
| |
|
| Source name |
Lung adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
shRNA: PSAT1 vector
cell line: CL1-0
cell type: Type II alveolar cell
|
| Treatment protocol |
Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
|
| Growth protocol |
Cells were cultured in 5% CO2 incubator at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from CL1-0 cells expressing PSAT1 vector
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 14, 2015 |
| Last update date |
Dec 29, 2020 |
| Contact name |
Michael Hsiao |
| E-mail(s) |
mhsiao@gate.sinica.edu.tw
|
| Phone |
+886-2-2789-1243
|
| Organization name |
Academia Sinica
|
| Department |
Genomics Research Center
|
| Street address |
Academia Road, Section 2, Nankang, Taipei, 115, Ta
|
| City |
Taipei |
| ZIP/Postal code |
049 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE75961 |
Expression data from PSAT1 overexpression in CL1-0 lung cancer cell line and PSAT1 knockdown in CL1-5 lung cancer cell line. |
|
