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Sample GSM1968998 Query DataSets for GSM1968998
Status Public on Aug 31, 2016
Title Microdissected E11.5 lung epithelial tip biological replicate 3
Sample type RNA
 
Source name Tip epithelial progenitor cells from E11.5 developing lung
Organism Mus musculus
Characteristics developmental stage: E11.5
cell type: Lung epithelial tip progenitor cells
cell isolation: Cells microdissected from embryonic lung
embryo genotype: Id2-CreER; Rosa26R-fGFP
Treatment protocol Lungs were dissected from E11.5 embryos and tip epithelium was microdissected following a 5 minute treatment with 16U/ml dispase. For E17.5 cells, pregnant females were injected with 150 micrograms per gram body weight at E16.5. E17.5 embryos were dissected and GFP+ lungs selected under GFP-dissecting microscope. Lungs were dissociated to single cells using trypsin/EDTA and GFP+ cells isolated by flow cytometry.
Growth protocol Timed matings of Id2-CreER/+heterozygotes with Rosa26R-fGFP/fGFP homozygotes. Noon on day of plug = E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA using Qaigen RNEasy Mini Kit as per manufacturer's instructions
Label Biotin
Label protocol Standard Affymetrix protocol using Ovation RNA Amplification Kit V2 and FL-Ovation Biotin V2 (NuGEN)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Data processing Bioconductor
 
Submission date Dec 09, 2015
Last update date Aug 31, 2016
Contact name Emma Louise Rawlins
E-mail(s) e.rawlins@gurdon.cam.ac.uk
Organization name University Of Cambridge
Department Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE75860 Expression data from mouse embryonic lung epithelial tip progenitor cells

Data table header descriptions
ID_REF
VALUE Log2 GC RMA signal

Data table
ID_REF VALUE
1446427_at 7.210790934
1436720_s_at 3.251071191
1424378_at 7.332074689
1417675_a_at 5.956365347
1436530_at 4.228439853
1429231_at 3.359115659
1431333_at 7.640513235
1428670_at 7.906183913
1457531_a_at 4.283836303
1431971_at 4.468322223
1428603_at 6.582756382
1454019_at 3.825894087
1431387_at 3.30190714
1454140_at 4.265521989
1428041_at 3.550915126
1432414_at 4.528135961
1460481_at 6.013374583
1455539_at 10.51565063
1458888_at 5.593335713
1419162_s_at 10.12370064

Total number of rows: 21204

Table truncated, full table size 480 Kbytes.




Supplementary file Size Download File type/resource
GSM1968998_E11_tip_C.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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