|
| Status |
Public on Dec 31, 2015 |
| Title |
Liver, 500 mg/kg, 4-Methylcyclohexanemethanol, Biological Rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, 500 mg/kg, 4-Methylcyclohexanemethanol
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Harlan Sprague-Dawley
tissue: Liver
compound: 4-Methylcyclohexanemethanol
dose: 500 mg/kg
time: 5 days
route: Gavage
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using TRI Reagent (Molecular Research Center Inc., Cincinnati, OH) according to manufacturer’s protocol
|
| Label |
Biotin
|
| Label protocol |
Purified total RNA (260/280 = 2.02 - 2.19) was hybridized to individual Affymetrix GeneChip® Rat Genome 230 2.0 plus microarrays according to the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com). Double stranded cDNA was synthesized from RNA using reverse transcriptase and an oligo-dT primer. Biotinylated RNA (labeled cRNA) served as the microarray target. cRNA was fragmented using heat and magnesium (Mg+2) reducing the cRNA to 25-200 base fragments to facilitate efficient and reproducible hybridization
|
| |
|
| Hybridization protocol |
Affymetrix GeneChips were hybridized labeled cRNA and then, washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual
|
| Scan protocol |
Standard Affymetrix procedures
|
| Data processing |
The raw microarray data (.CEL files) were normalized by applying the RMA algorithm provided by the genomics analysis tool, GeneSpring GX 12.6 (Agilent Technology, Foster City, CA); all microarrays from this series were normalized together
|
| |
|
| Submission date |
Dec 03, 2015 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Scott Auerbach |
| E-mail(s) |
AuerbachS@niehs.nih.gov
|
| Organization name |
Division of the National Toxicology Program at NIEHS
|
| Street address |
111 TW Alexander Drive
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE75657 |
5-Day Gavage Study of 4-Methylcyclohexanemethanol (CASRN: 34885-03-5) in Male Harlan Sprague Dawley Rats |
| GSE75658 |
West Virginia Chemical Spill |
|
